Fig 6.
Regulation of both ainS and luxR contributes to the effect of cAMP on luminescence. Maximal specific luminescence is shown for JB22 (lacIq-PA1/34-luxC), a luxR mutant complemented with constitutive luxR in trans (CL53 pJLB123), an ainS mutant biochemically complemented with 250 mM C8-HSL (NL60 C8-HSL), and a luxR ainS double mutant complemented with luxR in trans and 250 mM C8-HSL (NL62 pJLB123 C8-HSL). Cultures were grown in SWTO alone (gray bars) or with 10 mM cAMP added (black bars). Standard errors were calculated from two replicates. An asterisk indicates significant differences between cultures grown without and with cAMP as determined by a Student's t test (P ≤ 0.05).