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Domain architecture and activity of S. pneumoniae DacA. (A) Domain architecture of DacA. The numbers indicate the positions of amino acids in the full-length protein. TM, transmembrane domain. (B) Enzymatic activity of DacA. ATP was incubated with purified S. pneumoniae DacA, and the reaction mixture was separated using HPLC. ATP and c-di-AMP standards, as well as the reaction with purified B. subtilis DisA, were used as controls.
