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Cleavage of c-di-AMP and pApA by S. pneumoniae Pde1 and Pde2. (A) c-di-AMP at indicated concentrations was cleaved by Pde1 or Pde2 for 1 h. The enzymatic activity was determined as nmol c-di-AMP cleaved per mg protein per min. (B) pApA was cleaved by Pde2 for 10 min. Enzymatic activity was determined as μmol pApA cleaved by per mg protein per min. Note that different units are displayed for cleaved c-di-AMP (A) and pApA (B). The error bars indicate the SEM.
