Table 2.
Primera | Oligonucleotide sequence (5′ to 3′)b | Purposec |
---|---|---|
Mutagenesis | ||
Pr1097 | GAGATCTAGAACCGTTTGATTTTTAATGGATAATG | Janus cassette; For |
Pr1098 | GAGACTCGAGCCTTTCCTTATGCTTTTGGAC | Janus cassette; Rev |
Pr2874 | TGACGATAATCTTGTGACGG | dacA upstream; For |
Pr2875 | TTTTCTAGATTGATAGCTATCGTCC | dacA upstream; Rev |
Pr2876 | TTTCTCGAGTTTTAAAGAACGATTGC | dacA downstream; For |
Pr2877 | CCCTGAAATACGTGTGTCAC | dacA downstream; rev |
Pr2880 | TTTTGATCCTGATAATCACG | pde1 upstream; For |
Pr2881 | TTTTCTAGATGAATGATAATGGTGTCG | pde1 upstream; Rev |
Pr2882 | TTTCTCGAGTGGTGCTAATTCCTATAGC | pde1 downstream; For |
Pr2883 | GTGGTTCGTGACAATAGTAC | pde1 downstream; Rev |
Pr2900 | TTTCTCGAGATGATAATGGTGTCGTATTC | pde1 unmarked mutant; Rev |
Pr2901 | CATTTTCCCTTGTAAGATGAGG | pde2 upstream; For |
Pr2902 | TTTTCTAGACTTGTATAATGCACTCTCAG | pde2 upstream; Rev |
Pr2903 | TTTCTCGAGATGTAACCTTGTCAGAAGC | pde2 downstream; For |
Pr2904 | ACGCTTCGTAAGCTTGGTTG | pde2 downstream; Rev |
Pr2905 | TTTCTCGAGAAGTGGATAAGACTCCAAACG | pde2 unmarked mutant; Rev |
Overexpression | ||
Pr2895 | TTTCATATGTTGGGAAGAGCGACAGATTTC | dacA 89-271 aa; For |
Pr2879 | TTTGGATCCACAAGCAAAAAAGAGTGAGG | dacA 89-271 aa; Rev |
Pr2884 | TTTCCATGGAGATTTGCCAACAAATTTTAG | pde2 ORF; For |
Pr2885 | TTTAAGCTTGTTTTTAAGCAAGTTTTTTAAC | pde2 ORF; Rev |
Pr2960 | GCGGATCCAAGAAACTGAGAGTGCAT | pde151-657; For |
Pr2961 | GCCTCGAGTCATTCTTCTTTCTCCTTTTC | pde151-657; Rev |
Pr2962 | GCGGATCCACCAAGGAAGATGGTGATTTTG | pde1109-657; For |
The reverse primer of pde1109-657 was identical to pde151-657 Rev.
The restriction site is underlined if present in an oligonucleotide.
For, forward primer; Rev, reverse primer.