Table 2.

Primers used in this study

Primera	Oligonucleotide sequence (5′ to 3′)b	Purposec
Mutagenesis
Pr1097	GAGATCTAGAACCGTTTGATTTTTAATGGATAATG	Janus cassette; For
Pr1098	GAGACTCGAGCCTTTCCTTATGCTTTTGGAC	Janus cassette; Rev
Pr2874	TGACGATAATCTTGTGACGG	dacA upstream; For
Pr2875	TTTTCTAGATTGATAGCTATCGTCC	dacA upstream; Rev
Pr2876	TTTCTCGAGTTTTAAAGAACGATTGC	dacA downstream; For
Pr2877	CCCTGAAATACGTGTGTCAC	dacA downstream; rev
Pr2880	TTTTGATCCTGATAATCACG	pde1 upstream; For
Pr2881	TTTTCTAGATGAATGATAATGGTGTCG	pde1 upstream; Rev
Pr2882	TTTCTCGAGTGGTGCTAATTCCTATAGC	pde1 downstream; For
Pr2883	GTGGTTCGTGACAATAGTAC	pde1 downstream; Rev
Pr2900	TTTCTCGAGATGATAATGGTGTCGTATTC	pde1 unmarked mutant; Rev
Pr2901	CATTTTCCCTTGTAAGATGAGG	pde2 upstream; For
Pr2902	TTTTCTAGACTTGTATAATGCACTCTCAG	pde2 upstream; Rev
Pr2903	TTTCTCGAGATGTAACCTTGTCAGAAGC	pde2 downstream; For
Pr2904	ACGCTTCGTAAGCTTGGTTG	pde2 downstream; Rev
Pr2905	TTTCTCGAGAAGTGGATAAGACTCCAAACG	pde2 unmarked mutant; Rev
Overexpression
Pr2895	TTTCATATGTTGGGAAGAGCGACAGATTTC	dacA 89-271 aa; For
Pr2879	TTTGGATCCACAAGCAAAAAAGAGTGAGG	dacA 89-271 aa; Rev
Pr2884	TTTCCATGGAGATTTGCCAACAAATTTTAG	pde2 ORF; For
Pr2885	TTTAAGCTTGTTTTTAAGCAAGTTTTTTAAC	pde2 ORF; Rev
Pr2960	GCGGATCCAAGAAACTGAGAGTGCAT	pde151-657; For
Pr2961	GCCTCGAGTCATTCTTCTTTCTCCTTTTC	pde151-657; Rev
Pr2962	GCGGATCCACCAAGGAAGATGGTGATTTTG	pde1109-657; For

^aThe reverse primer of pde1_109-657 was identical to pde1_51-657 Rev.

^bThe restriction site is underlined if present in an oligonucleotide.

^cFor, forward primer; Rev, reverse primer.