Table 2.
Sensitivity of ainR-dependent effects on the Pqrr-lacZ reporter to different AHL molecules
| AHL | AHL concn (nM) |
|
|---|---|---|
| EC50a | Minimum stimulatory doseb | |
| C6-AHL | 114 | 1,000 |
| 3OC6-AHL | 29 | 100 |
| C7-AHL | 11 | 10 |
| 3OC8-AHL | 8 | 1 |
| 3OHC8-AHL | 0.8 | 1 |
| C10-AHL | 476 | 1,000 |
a
EC50s were generated from dose-response assays using NL63 (ΔainS luxI) harboring the Pqrr-lacZ reporter pHK20 grown in SWTO. AHL was supplemented in 10-fold steps over a range of 1 μM to 1 pM. Data are from a single representative experiment of three independent experiments.
b
Lowest tested AHL concentration that elicited a significantly lower (P < 0.05) Pqrr-lacZ activity than the treatment lacking AHL.