Fig 1.
The NrrF promoter binds with high affinity to the gonococcal Fur protein. EMSA reactions were carried out using 25 nM each respective DNA target with increasing concentrations (μM) of gonococcal Fur protein (given below each lane). Promoter controls included NGO1779 (fur) and the nonspecific DNA fragment NGO1809 (NS), which does not contain a Fur box. (A, B, and C) Representative EMSA gels of nrrF, fur, and nonspecific NGO1809, respectively. (D) The amounts of DNA in bound and unbound bands were measured and were plotted to estimate the percentage of binding. The KD was defined as the concentration of Fur required for a 50% shift of the target DNA and is representative of two separate experiments.