Fig 1.

High concentrations of phosphate inhibit gene transduction. The transduction frequencies of SB1003 culture supernatants are shown. (A) Transduction frequencies of cultures grown in YPSm medium (None) or YPSm supplemented with 10 mM KPO₄, NaPO₄, or MOPS; (B) transduction frequencies of cultures grown in RCVm medium containing various concentrations of KPO₄ (0.5, 2.0, 5.0, and 10 mM); (C) pH of culture supernatant at harvest. Error bars represent the standard deviations of three biological replicates. Samples were normalized to the culture turbidity at 660 nm (A and B).