Fig 3.

Release of RcGTA is inhibited by high phosphate concentrations. Western blots of culture supernatant and cell pellet fractions probed using RcGTA capsid protein antiserum are shown. (A) Western blots of cells grown in YPSm complex medium (None) and YPSm supplemented with 10 mM KPO₄, NaPO₄, KSO₄, KNO₃, or MOPS, as indicated; (B) Western blots of cells grown in RCVm defined medium containing the indicated concentrations of KPO₄. Samples were normalized to the culture turbidity at 660 nm.