Table 2.
Primer sequences
| Name | Sequence | Purpose or description of sequence |
|---|---|---|
| CckA-F | TAAGTAGTCGACGATCTGGTGCTGGT | Amplification of cckA::KIXX fragment |
| CckA-R | ACAATGGTCGACACGCTTTCGCACAG | |
| CckAComp-F | ATATTCTAGAGGTGCTGGTCGATGCGCCCT | cckA complementation plasmid pRCckA; XbaI (italic) and HindIII (bold) sites are indicated |
| CckaComp-R | ATATAAGCTTCTGCAGCCCGAGACCGAGGC | |
| GTA2.6 | GAACCGGATCCATCGCCAGGG | Plasmids p601-g64 and p601-g61; the BamHI (underlined) site is indicated |
| GTA4 | CGCCTGCAGCAACCCTGAATATAGC | Plasmid p601-g64; the PstI (bold) site is indicated |
| GTA1 | ACGCTTCAAGCTGCAGATAAGGCATG | Plasmid p601-g61; the PstI (bold) site is indicated |
| GTA5 | GATGCGGCTGCAGACCGATCC | Plasmid p601-g15; the PstI (bold) site is indicated |
| GTA2.1 | CTCCAGCGGATCCACCGGAGG | Plasmid p601-g15; the BamHI (underlined) site is indicated |
| 555-F | ATATCTGCAGGCGTGCTGCCCGACCTCTTT | Plasmid pXCA-555; the PstI (bold) and BamHI (underlined) sites are indicated |
| 555-R | ATATGGATCCATCCGATCCCCCTTGGCTGAG | |
| GSP1 | CTTGTCTGCGAAGTTTTTCA | ORF g1 RACE |
| GSP3 | GCAATTTCCCGATAAAGCTCCTC | |
| 556-F | ATATCCATGGGGCTGATCGGGACGAT | Plasmid pI556 and pI556Gm; the NcoI (bold) and BamHI (underlined) sites are indicated |
| 556-R | ATATGGATCCTGCCTTTGCGGTGCCCGAAAA | |
| 555C-F | ATATCCATGGGATCTGTCTACGAGATTGC | Plasmid pET-555C; the NcoI (bold) and XhoI (underlined) sites are indicated |
| 555C-R | ATATCTCGAGGCCCCATGCCGCCACCCG | |
| aacC1-F | ATATCCCGGGTTGACATAAGCCTGTTCGGT | aacC1 gene conferring Gmr; the XmaI (bold) sites are indicated |
| aacC1-R | ATATCCCGGGCTTGAACGAATTGTTAGGTG |