Table 2.
Published plasmids used in this work
| Plasmid | Antibiotic resistance | Descriptiona | Reference or source |
|---|---|---|---|
| CFP B | Apr | Cre fusion plasmid; cre from phage P1 cloned into the NheI and SalI site of pBR322 | 9 |
| pCIG1077 | Apr | pKK223-3::PABCtrwA-trwL | 34 |
| pCMS1 | Apr | trwC with XhoI site before stop codon | 14 |
| pCre-TrwC | Apr | R388 trwC encoding residues 2 to 966 in CFP B | 35 |
| pHP159 | Gmr | pBBR6::oriT trwABC::eGFP→ | 14 |
| pHP161 | Gmr | pBBR6::oriT trwABC::eGFP← | 14 |
| pHP178 | Gmr, Cmr | pHP161ΔtrwB::Cm | 14 |
| pHP181 | Gmr | pBBR6::oriT trwAB::eGFP | 14 |
| pLA24 | Gmr | pHP159::trwC::BID | 14 |
| pPG104 | Gmr | Source of bepD-BID sequence | 13 |
| pSU1395 | Apr, Kmr | pHG327::oriT trwABC with Tn5tac1 in trwC | 20 |
| pSU1443 | Kmr | pSU1425::Tn5tac1 in trwB | 20 |
| pSU1445 | Kmr | pSU1425::Tn5tac1 in trwC | 20 |
| pSU4058 | Apr | pHG327::trwL-trwD | 36 |
a
Arrows indicate the orientation of the eGFP cassette.