Table 5.
Net charges and hydrophobicities of the C termini of TrwC derivatives
| Plasmid | TrwC description | C-terminal 183 aa |
C-terminal 32 aa |
C-terminal 20 aa |
|||
|---|---|---|---|---|---|---|---|
| Charge | GRAVYa | Charge | GRAVY | Charge | GRAVY | ||
| pHP161 | 966 aa (wild type) | +7 | −1.039 | +3 | −2.416 | +1 | −1.705 |
| pYG2 | 934 aa + 32-aa C-terminal tail | +10 | −0.738 | +7 | −0.891 | +6 | −1.320 |
| pAA12 | 966 aa + 25-aa C- terminal tail (RalF TS) | +8 | −1.068 | +2 | −1.278 | +1 | −1.530 |
| pEF18 | 966 aa + 107-aa C-terminal tail (VceC B. suis TS) | +18 | −0.868 | +5 | 0.056 | +5 | −0.810 |
| pEF19 | 966 aa + 115-aa C-terminal tail (VceC B. abortus TS) | +26 | −0.969 | +9 | −0.769 | +8 | −1.440 |
| pAA15 | 934 aa + 183-aa C-terminal tail (BID) | +5 | −1.141 | +2 | −2.013 | +2 | −1.740 |
| pLA24 | 966 aa + 183-aa C-terminal tail (BID) | +5 | −1.141 | +2 | −2.013 | +2 | −1.740 |
a
GRAVY (grand average of hydropathicity) is an index that indicates the solubility of proteins; positive is hydrophobic, and negative is hydrophilic.