Fig 4.

Germination of C. perfringens wild-type and mutant spores with Ca-DPA (A) and dodecylamine (B). Heat-activated spores of strains SM101 (wild-type) (◆), DPS119 (gerKA) (▲), DPS122 (gerKC) (□), DPS124 (gerKB gerAA) (●), and DPS122(pSB18) (gerKC mutant complemented with wild-type gerKA-gerKC) (△) were germinated with Ca-DPA and dodecylamine. For Ca-DPA, changes in OD₆₀₀ were measured as described in Materials and Methods, and for dodecylamine, germination was monitored by measurement of DPA release, as described in Materials and Methods. Error bars represent standard deviations.