Fig 7.
Quantitation of the GerKC level in spores of C. perfringens strain SM101. Various amounts of P100 pellet IM fractions (lanes 1 to 3) and pellet fractions of decoated, disrupted spores obtained by centrifugation at 14,000 × g (lanes 4 and 5) and various amounts of purified GerKC antigen (lanes 6 to 8) were run on SDS-polyacrylamide gels, followed by Western blot analysis using anti-GerKC antibody. The amounts of samples run are shown above the lanes as either the percentage of the total membrane protein or pellet fraction protein from 8.4 × 109 spores (lanes 1 to 5) or the amount (in ng) of GerKC antigen (lanes 6 to 8). The numbered lines to the left of the figure denote the migration positions of molecular mass markers (in kDa). All lanes are from the same gel, but lanes 4 and 5 were moved from their original position to the right of the GerKC antigen lanes.