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Protein blot analysis of BglAx. (A) Wild type (WT) and five Cel− mutants. The gene disrupted in each mutant is indicated in parentheses. (B) Mutants II-23, II-23CE (complemented mutant II-23), II-23OE (WT transformed with a functional copy of crp-fnr in pUCD2), and bglAx− (a bglAx knockout mutant). An anti-BglAx antibody was used for both blots.
