Table 3.
Constructs used in complementation experiments
| Construct | Promoter (bp)a | Coding sequence | Length of gene + 3′-UTR (bp) |
|---|---|---|---|
| pUCD2::acsp::acsAB::acsC | Promoter of acs operon (221) | acsAB::acsC | 8,237 + 93 |
| pUCD2::acsp::acsC | Promoter of acs operon (221) | acsC | 3,588 + 93 |
| pUCD2::cmcAxp::cmcAx::ccpAx | Promoter of cmcAx-ccpAx operon (504) | cmcAx::ccpAx | 2,087 + 111 |
| pUCD2::dgc1 | dgc1 promoter (108) | dgc1 | 1,719 + 108 |
| pUCD2::crp-fnr | crp-fnr promoter (136) | crp-fnr | 744 + 106 |
a
The base pair number in parentheses denotes the size of the fragment used in each construct and not the actual size of the promoter.