Table 3.

Incorporation of d[¹⁴C]alanine into various cellular fractions of B. pertussis WT and Δdra strains

Fraction or treatment	cpm (100 μg protein)a		P value
	WT	Δdra mutant
Total membranes	1,056 ± 7	484 ± 36	<0.005
Boiled membranes	48 ± 6	41 ± 7	NS
2% Triton X-100
Initial counts (total membranes)	1,208 ± 144	502 ± 62	<0.05
Supernatant	330 ± 42	247 ± 88	NS
Pellet	812 ± 66	384 ± 129	<0.05
8% SDS
Initial counts (total membranes)	2,137 ± 30	781 ± 45	<0.001
Supernatant	1,664 ± 24	632 ± 60	<0.001
Pellet	328 ± 11	135 ± 28	<0.001
SDS-EDTA boiling
Total membranes	1,056 ± 71	484 ± 36	<0.005
Supernatant	1,110 ± 62	482 ± 219	<0.05
Pellet	108 ± 66	85 ± 66	NS
Proteinase K
Initial counts (total membranes)	1,056 ± 71	484 ± 37	<0.005
Supernatant	587 ± 132	182 ± 50	<0.05
Pellet	184 ± 26	103 ± 22	<0.05

^aRepresentative data from one of at least two to three independent experiments are shown.

NS, not significant.