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Gel retardation assay of FeaR CTD binding to the tynA promoter. A fragment from the ytfE promoter was used as a negative control. The labeled DNAs were tynA (lanes 1 to 5) and ytfE (lanes 6 to 10), and the protein concentrations were 0 (lanes 1 and 6), 250 nM (lanes 2 and 7), 500 nM (lanes 3 and 8), 750 nM (lanes 4 and 9), and 1,000 nM (lanes 5 and 10).
