Fig 5.

Phylogenomics can be used to predict small-molecule ligands for TFRs of unknown function. (A) The TFR of unknown function SSQG_00958 is predicted to bind a polyether ionophore based on grouping with MonRII and SchR3. (B) TFRs encoded in the biosynthesis clusters for macrolactam antibiotics cluster together, leading to the prediction that all of the TFRs in this group interact with macrolactam antibiotics. (C) AefR may recognize a phytosterol based on clustering with BreR. (Adapted from reference 25.)