Table 1.
E. coli gene targets for the real-time PCR array
Gene (ORF name if chromosomal)a | Encoded protein or family effector | Genetic support (mobile element)a |
---|---|---|
ureD (Z1142) | Urease-associated protein UreD | OI-43 and OI-48 |
espV (Z1387) | AvrA family effector | OI-44 |
espK (Z1829) | Leucine-rich repeats | OI-50 (prophage CP-933N) |
espN (Z1824) | Hypothetical protein | OI-50 (prophage CP-933N) |
(Z2098) | Hypothetical protein | OI-57 |
espM1 (Z2565) | Non-LEE-encoded type III effector | OI-71 |
Nomenclature of ORFs and mobile elements refers to the sequence of E. coli O157:H7 EDL933 (GenBank accession no. AE005174).