Table 2.
Primers and probes for the real-time PCR array
| Target gene (ORF name if chromosomal)a | Primer |
Probe sequence (5′ to 3′)b | Location within GenBank sequence AE005174 (nt range) | |
|---|---|---|---|---|
| Forward | Reverse | |||
| ureD (Z1142) | GCAATAATTGACTCTGATTGCC | 1078824–1078845 | ||
| GCTGCTGCGGTAAAATTTACT | 1078892–1078872 | |||
| [FAM]-TACGCTGATCACCATGCCTGGTGC-[BHQ1] | 1078847–1078870 | |||
| espV (Z1387) | TCAGGTTCCTCGTCTGATGCCGC | 1295446–1295424 | ||
| CTGGTTCAGGCCTGGAGCAGTCC | 1295360–1295382 | |||
| [FAM]-CTTGCAACACGTTACGCTGCCGAGTATT-[BHQ1] | 1295422–1295395 | |||
| espK (Z1829) | GCAGRCATCAAAAGCGAAATCACACC | 1673422–1673397 | ||
| TCGTTTGGTAACTGTGGCAGATACTC | 1673312–1673338 | |||
| [FAM]-ATTCAGATAGAAGAAGCGCGGGCCAG-[BHQ1] | 1673395–16673370 | |||
| espN (Z1824) | GACATATTTGTTTATGTCATCAGGAGCGG | 1666890–1666918 | ||
| CCTCAGGATATGGATGGCCTACTGGC | 1667017–1666992 | |||
| [FAM]-AATGCTCTCGGCAATCGAATCCTTGACTC-[BHQ1] | 1666991–1666963 | |||
| Z2098 | CTGAAAAGAGCCAGAACGTGC | 1888173–1888193 | ||
| TGCCTAAGATCATTACCCGGAC | 1888308–1888287 | |||
| [HEX]-TAACTGCTATACCTCCGCGCCG-[BHQ1] | 1888286–1888265 | |||
| espM1 (Z2565) | GCGCTCTATCCGCTTTAATGTTAAC | 2275378–2275354 | ||
| CCATCCATGAATATCTTTAGTACTCTGC | 2275291–2275318 | |||
| [FAM]-TGCTTACCGTCTCCAGTATACAGCCGCT-[BHQ1] | 2275320–2275347 | |||
a
Numbering as in E. coli O157:H7 EDL933 (GenBank accession no. AE005174).
b
FAM, 6-carboxyfluorescein; BHQ1, black hole quencher 1; HEX, 5′-hexachlorofluorescein.