Table 2.
Target gene (ORF name if chromosomal)a | Primer |
Probe sequence (5′ to 3′)b | Location within GenBank sequence AE005174 (nt range) | |
---|---|---|---|---|
Forward | Reverse | |||
ureD (Z1142) | GCAATAATTGACTCTGATTGCC | 1078824–1078845 | ||
GCTGCTGCGGTAAAATTTACT | 1078892–1078872 | |||
[FAM]-TACGCTGATCACCATGCCTGGTGC-[BHQ1] | 1078847–1078870 | |||
espV (Z1387) | TCAGGTTCCTCGTCTGATGCCGC | 1295446–1295424 | ||
CTGGTTCAGGCCTGGAGCAGTCC | 1295360–1295382 | |||
[FAM]-CTTGCAACACGTTACGCTGCCGAGTATT-[BHQ1] | 1295422–1295395 | |||
espK (Z1829) | GCAGRCATCAAAAGCGAAATCACACC | 1673422–1673397 | ||
TCGTTTGGTAACTGTGGCAGATACTC | 1673312–1673338 | |||
[FAM]-ATTCAGATAGAAGAAGCGCGGGCCAG-[BHQ1] | 1673395–16673370 | |||
espN (Z1824) | GACATATTTGTTTATGTCATCAGGAGCGG | 1666890–1666918 | ||
CCTCAGGATATGGATGGCCTACTGGC | 1667017–1666992 | |||
[FAM]-AATGCTCTCGGCAATCGAATCCTTGACTC-[BHQ1] | 1666991–1666963 | |||
Z2098 | CTGAAAAGAGCCAGAACGTGC | 1888173–1888193 | ||
TGCCTAAGATCATTACCCGGAC | 1888308–1888287 | |||
[HEX]-TAACTGCTATACCTCCGCGCCG-[BHQ1] | 1888286–1888265 | |||
espM1 (Z2565) | GCGCTCTATCCGCTTTAATGTTAAC | 2275378–2275354 | ||
CCATCCATGAATATCTTTAGTACTCTGC | 2275291–2275318 | |||
[FAM]-TGCTTACCGTCTCCAGTATACAGCCGCT-[BHQ1] | 2275320–2275347 |
Numbering as in E. coli O157:H7 EDL933 (GenBank accession no. AE005174).
FAM, 6-carboxyfluorescein; BHQ1, black hole quencher 1; HEX, 5′-hexachlorofluorescein.