Minimum spanning tree (Manhattan) produced using 15 loci on 10 PCR ribotype 017 isolates from Xiangya Hospital, China. Each node represents a single MLVA profile and the number of isolates with that profile is indicated by the node size. Nodes are coded by ward as follows: neurology (diagonal lines), spinal surgery (horizontal lines), respiratory (grid lines), hematology (shaded and checkerboard lines), and cardiology (checkerboard). Numbers between the circles are summed tandem repeat differences (STRDs); the unnumbered lines represent 1 STRD and numbers within the circles are the patient numbers. Gray shading identifies isolates belonging to a cluster with STRDs of <4.