Fig 5.

Association of Shy1 and Cox15 with COA complexes. (A) Coimmunoprecipitation of Coa3 from digitonin-solubilized mitochondria isolated from wild-type (WT) and shy1Δ cells were analyzed by SDS-PAGE and Western blotting (total, 8%; eluate, 100%). (B) Coimmunoprecipitation experiments, as for panel A, were performed from wild-type and cox15Δ mitochondrial extracts. (C and D) Mitochondria from wild-type, cox1⁻, and cox2⁻ cells were subjected to Coa3 (C) or Coa1 (D) immunoprecipitation. (E) Purification of Shy1^ProtA-containing complexes by IgG chromatography from wild-type mitochondria compared to results for mitochondria lacking mitochondrial DNA (rho⁰) (total, 3%; eluate, 100%). The asterisk indicates a cross-reactive signal. (F) BN gel of Shy1^ProtA purified complexes from wild-type and rho⁰ mitochondria.