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. 2013 Oct;33(20):4106–4115. doi: 10.1128/MCB.00144-13

Fig 3.

Fig 3

Mbd2 knockdown or transduction modulates Foxp3 expression and Treg function. (a) Knockdown of Mbd2 decreased Treg expression of Foxp3 and Foxp3-dependent genes compared to the effects of scrambled siRNA or control lipofectamine. Data are shown as expression levels relative to that seen using specific Mbd2-targeting siRNA (means ± SD, *, P < 0.05; **, P < 0.01). (b) Viral transduction of Mbd2−/− Treg cells with Mbd2 increased expression of Foxp3, CTLA4, and TGF-β and IL-10 genes and decreased IL-6 gene expression. Data are shown as expression levels relative to empty vector (EV control) (means ± SD, *, P < 0.05; **, P < 0.01). (c) Effects of Mbd2 viral transduction on Treg-suppressive function in vitro; the proportion of proliferating cells is shown in each panel, and data are representative of 3 separate assays.