Fig 8.

Overexpressed GFP-Mim1 is mislocalized to the ER in djp1Δ cells. (A) Fluorescence images of wt yeast cells expressing GFP-Mim1 and Cherry-Fis1 (as a mitochondrial marker protein). (B) GFP-Mim1 was transformed into yeast cells deleted in each of the cytosolic Hsp40 cochaperones, and fluorescence images were taken. (C) Fluorescence images of wild-type or djp1Δ yeast cells expressing GFP-Mim1. (D) Yeast cells expressing Djp1 under the control of the GAL1 promoter were transformed with the GFP-Mim1 expressing vector. Cells were grown on synthetic medium containing glucose (to repress expression of Djp1), raffinose (to enable low levels of Djp1 expression), or galactose (to overexpress Djp1), and fluorescence images were taken.