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. 2013 Oct;33(20):3994–4007. doi: 10.1128/MCB.00623-13

Fig 2.

Fig 2

miR-23a and miR-27a upregulated β-like globin genes in human primary erythroid cells. (A) qPCR analysis of miR-23a and miR-27a expression in lentivirus-infected human CD34+ HPCs after Epo induction for 4, 8, and 11 days. (B) qPCR analysis of γ-globin gene expression normalized with GAPDH in lentivirus-infected human CD34+ HPCs at different induction days. (C) Western blot analysis of γ-globin expression in miR-23a- and miR-27a-overexpressing CD34+ HPCs after Epo induction. (D) Immunostaining of human γ-globin in lentivirus-infected human CD34+ HPCs after Epo induction for 11 days. DAPI, 4′,6-diamidino-2-phenylindole. (E) FACS analysis of GFP-positive cells and CD71/CD235a double-positive cells among them at 11 days after Epo induction of CD34+ HPCs. (F) May-Grünwald-Giemsa staining of lentivirus-infected human CD34+ HPCs after Epo induction for 8 and 11 days. Student's t test (two-tailed) was performed to analyze the data from the experiments in triplicate. *, P < 0.05; **, P < 0.01; ***, P < 0.001.