Expression of rtxA, rtxB, rtxH, plp, and vah1 determined by qRT-PCR analysis of V. anguillarum wild-type (M93Sm), hns mutant (hns−), and hns-complemented mutant (hns+) strains during logarithmic (Log)- and stationary (Sta)-phase growth. The data presented are representative of two independent experiments. Each value is the average for three replicates. Asterisks represent P values of <0.05 between two bracketed strains. Error bars represent 1 standard deviation.