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. 2013 Oct;81(10):3566–3576. doi: 10.1128/IAI.00506-13

Fig 6.

Fig 6

(A) Hemolytic activity of V. anguillarum wild-type (M93Sm), hns mutant (hns−), hns hlyU double mutant (hns−/hlyU−), and hlyU-complemented hns hlyU double mutant (hns−/hlyU+) strains on TSA-5% sheep blood agar after 24 h at 27°C. (B) Expression of rtxA, rtxB, plp, and vah1 determined by qRT-PCR analysis of V. anguillarum wild-type (M93Sm), hns mutant (hns−), hns hlyU double mutant (hns−/hlyU−), and hlyU-complemented hns hlyU double mutant (hns−/hlyU+) strains during logarithmic (Log)- and stationary (Sta)-phase growth. The data presented are representative of two independent experiments. Each value is the average for three replicates. Error bars represent 1 standard deviation.