Fig 7.

Capillary electrophoresis of FAM-labeled DNA probe 1 (A) and probe 2 (B), specific for the vah1-plp intergenic region, and probe 4 (C), specific for the rtxB-rtxH intergenic region, from DNase protection assays in the presence (black lines) and absence (gray lines) of rH-NS, demonstrating that H-NS binds to specific sequences in the vah1-plp and rtxB-rtxH intergenic regions and protects against DNase I digestion. DNA probes were prepared and labeled with FAM, incubated with rH-NS (0 or 3 μM) followed by DNase I, and then analyzed by DNA fragment analysis as described in Materials and Methods. The binding region sequences are indicated by double black underlining. The underlined DNA fragments indicate those that are more common in the presence of rH-NS (black lines) than in its absence (gray lines). The location of each probe is shown in Fig. S1 in the supplemental material.