Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or feature(s) | Reference or source |
|---|---|---|
| Strains | ||
| V. anguillarum strains | ||
| M93Sm | Spontaneous Smr mutant of M93 (serotype J-O-1); virulent | 31 |
| S305 | hlyU mutant; Smr Cmr | 6 |
| M114 | hns mutant; Smr Cmr | This study |
| M116 | hns-complemented strain; Smr Cmr Tetr | This study |
| ES114 | hns hlyU double mutant; Smr Cmr Kmr | This study |
| ES115 | hns hlyU double mutant complemented with hlyU; Smr Cmr Tetr Kmr | This study |
| ES116 | hns hlyU double mutant complemented with hns; Smr Cmr Tetr Kmr | This study |
| E. coli strains | ||
| Sm10 | thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λ pir) Kmr | 39 |
| M15 | Nals Strs Rifs thi lac ara+ gal+ mtl F− recA+ uvr+ lon+ (pREP4; Kmr) | Qiagen |
| D112 | E. coli Sm10 with pDM4-hns5′-Kan-hns3′; Cmr Kmr | This study |
| Plasmids | ||
| pNQ705-1 | Cmr; suicide vector with R6K origin | 40 |
| pNQ705-hns | Used for hns insertional mutation | This study |
| pSUP202 | E. coli-V. anguillarum shuttle vector | 39 |
| pSUP202-hlyU | Used for complementation of hlyU | 6 |
| pSUP202-hns | Used for complementation of hns | This study |
| pDM4 | Cmr Kanr SacBCr; suicide vector | 18 |
| pDM4-hns5′-Kan-hns3′ | Used for hns deletion mutation | This study |
| pQE-30 UA | Expression vector with N-terminal His6 tag | Qiagen |
| pQE-30 UA/H-NS | Used for expression of rH-NS | This study |