Atypical EPEC 1551-2 interactions with HeLa cells. (A, B, and C) Light microscopy images demonstrating the differences in the adherence patterns between 1551-2 (LA), the derivative eae mutant (DA), and the complemented strain (LA), respectively (original magnification, ×1,000). (D) Quantitative adherence assay demonstrating a reduction of approximately 33.7% in adherence of 1551-2 eae::Km compared with the wild-type strain. *, P < 0.05; #, significantly more adherent than 1551-2 eae::Km (P < 0.05). (E and F) SEM images showing the presence or absence of AE lesions (arrows) after 6 h of contact by 1551-2 and 1551-2 eae::Km, respectively. Bar = 2 μm.