Fig 4.

Contribution of T3SS to the adherence of aEPEC 1551-2 to HeLa cells. (A and B) Adherence of the T3SS-ATPase-deficient mutant (1551-2 escN::Km) and the complemented strain after 6-h assay (original magnification, ×1,000). (C) Quantitative comparison of adherence between the indicated strains demonstrating that adherence of 1551-2 to HeLa is dependent on a functional T3SS. ***, significantly less adherent than 1551-2 (P < 0.005); ###, significantly more adherent than the 1551-2 escN::Km mutant (P < 0.001).