Table 1.
Escherichia coli strains and plasmids used in this study
| E. coli strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| 1551-2 | aEPEC (ONT:H−) isolated from a child with diarrhea in Brazil (Nalr) | 13 |
| 1551-2 eae::Km | 1551-2 harboring the suicide vector pJP5603 inserted into the eae gene (Nalr Kmr) | 24 |
| 1551-2 ΔfimA | fimA::zeo (Nalr Zeor) | This study |
| 1551-2 sslE::Km | 1551-2 harboring the suicide vector pJP5603 inserted into sslE gene (Nalr Kmr) | This study |
| 1551-2 escN::Km | 1551-2 harboring the suicide vector pJP5603 inserted into escN gene (Nalr Kmr) | This study |
| 1551-2 eae::Km Δtir | Double intimin/Tir mutant in aEPEC 1551-2 (Nalr Kmr Zeor) | This study |
| 1551-2 eae::Km ΔfimA | Double intimin/T1P mutant in aEPEC 1551-2 (Nalr Kmr Zeor) | This study |
| 1551-2 eae::Km ΔespA | Double intimin/EspA mutant in aEPEC 1551-2 (Nalr Kmr Zeor) | This study |
| Plasmids | ||
| pEscN | pACYC184 vector harboring the escN gene from EPEC prototype E2348/69 | 31 |
| pJP5603 | 3.1-kb R6K-based suicide vector, Kmr | 29 |
| pKOBEG-Apra | Red recombinase system plasmid, Aprar | 44 |
| 1711-4ΔfliC | Source of zeocin cassette | 44 |
| pGEM-T Easy | Cloning vector | Promega |
| pBAD/Myc-His A | Cloning vector | Invitrogen |
| pFimA | pBAD/Myc-His A vector harboring the fimA gene from aEPEC 1551-2 | This study |
| pInt | pBAD/Myc-His A vector harboring the eae gene from aEPEC 1551-2 | This study |
| pEspA | pBAD/Myc-His A vector harboring the espA gene from aEPEC 1551-2 | This study |
a
Nalr, nalidixic acid resistant; Kmr, kanamycin resistant; Zeor, zeocin resistant; Aprar, apramycin resistant.