Fig 1.

YT cell anticryptococcal activity is lipid raft dependent and accompanied by early tyrosine phosphorylation of a 60-kDa protein. (A) YT cells were left untreated or pretreated with H₂O (vehicle control, 5% [vol/vol]) or 2 mM MBCD for 30 min. They were then washed several times in serum-free medium and incubated with C. neoformans (Crypto) for 24 h. CFU counts were determined at 0 and 24 h. **, P < 0.01; ***, P < 0.001. Data are representative of three experiments. (B) YT cells were left unstimulated or stimulated with C. neoformans for 1 to 30 min. Immunoblotting was performed with anti-phospho-Tyr (p-Tyr; clone 4G10). Data are representative of three experiments.