Fig 4.

Kinetics of biofilm formation and detachment. The wild-type and smcR mutant biofilms were grown on the microtiter plate walls and quantitated using CV staining (Materials and Methods) at the indicated time points over 40 h of incubation at 30°C. Error bars represent the SEM. Mean and SEM values were calculated from three independent experiments, and the P value for the slope of the regression was less than 0.0001. WT(pRK415), wild type; HS03(pRK415), smcR mutant; HS03(pHS105), complemented strain.