Fig 7.

SmcR-regulated genes possibly involved in biofilm development. Nine genes possibly involved in biofilm development were chosen from the pool of the SmcR regulon members predicted by a microarray analysis. The ORF spots that showed 1.58-fold or greater difference in expression levels with a P value of ≤0.05 were considered to be regulated by SmcR. Regulation of their transcription by SmcR was confirmed by qRT-PCR. Each column represents the mRNA expression level in biofilm cells of the smcR mutant relative to that of the isogenic wild type. Locus tags are based on the database of the V. vulnificus MO6-24/O genome, which was retrieved from GenBank (accession numbers CP002469.1 and CP002470.1), and the products of the nine genes are listed on the right. The expression levels of the genes are the means ± SEM of results from three independent experiments. MCP, methyl-accepting chemotaxis protein.