Table 2.
Oligonucleotides used for quantitative real-time PCR
| Locus taga | Oligonucleotide sequence (5′–3′)b |
|
|---|---|---|
| Forward | Reverse | |
| VVMO6_00535 | CACACACTTCACCACGCTCAATG | AACATCGCCAACATCACCAACG |
| VVMO6_00513 | CGATGGAAGATGTGTTGAAAGT | TCCTGCTGCCAGAATGTT |
| VVMO6_04125 | GAAAAAGATATTACTAACTTGTTTA | TTCCTGACCACCTCAGACAG |
| VVMO6_04124 | TACGGGAATCGCTATGGCT | TCATGGCTGATCTGAATATCTAAAC |
| VVMO6_02022 | GCGGATGCACTGTGTCATT | TCAGAAGCCGTTTGCCTG |
| VVMO6_02023 | CCGCCTCCTTTGAACCAG | CGGAATGTTACCATCACACG |
| VVMO6_03339 | GCTAGATATCGAGCTAACCAGAC | CAATCTTGCGGAAAATATTATAAA |
| VVMO6_00926 | GACGTTTGAGTTTGGTTTACGTAGC | CCACAACTGAAGGTGATCGGG |
| VVMO6_03635 | GGTTGGCTTATCCATTGATGA | TTGTTGATGCTTGTAGTTAGTGA |
| VVMO6_03732 | ATGGCATTGAGTGTATCCCTGTATT | GCTTTAGTGTCCAGCGCAGTAATAC |
| VVMO6_04095 | ATCGTCGTGGTTCTGGAA | TGTCTTCGTCCGTGTCAT |
| VVMO6_02139 | TGCACATTTTGGAAAACAGTCATC | TGCTGACCATTTCCACCATGT |
| VVMO6_00361 | TTTGAAAGATTTGGGTGTGGATTAC | CTTGTCCACCATGTATGCCTAGCTC |
| VVMO6_04435 | TTTTGGTGAGTCAGAGCGTTGC | CTGAAATGCCCGTAACGTGGT |
| VVMO6_04367 | CAATATTGCAGCTTTAACGTCACAC | AACCGACCACAAGCTCTTGGG |
a
Locus tags are based on the database of the V. vulnificus MO6-24/O genome (GenBank accession numbers CP002469.1 and CP002470.1) as presented in Fig. 7 (except VVMO6_00535 for smcR, VVMO6_00513 for luxS, VVMO6_04125 for luxP, VVMO6_04124 for luxQ, VVMO6_02022 for luxU, and VVMO6_02023 for luxO, which were used in Fig. 6).
b
The oligonucleotides were designed using the genomic sequence of V. vulnificus MO6-24/O (55).