Fig 2.
Phenotype of gastric DC-LAMP+ DCs from H. pylori-infected subjects. Gastric DCs from uninfected (Hp−) and H. pylori-infected (Hp+) individuals were analyzed by flow cytometry for expression of HLA-DR and DC-LAMP (A) or CD11c and DC-LAMP (B). The lower dot plots show staining with an isotype control. Numbers by the gates indicate cell frequencies among all live (7AAD−) cells. (C) Percentage of DC-LAMP+ DCs, gated as described for panels A and B, in the gastric lamina propria of uninfected and H. pylori-infected subjects. Symbols represent individual values, which were calculated from several stainings in each individual, and horizontal lines indicate the median. All staining with the isotype control was subtracted from the DC-LAMP staining. (D) Flow cytometric analysis of the phenotype of gastric DC-LAMP+ HLA-DRhigh cells identified as described for panels A and B. Thick lines, staining with the indicated antibody; dotted lines, staining with isotype controls. The data shown are from two H. pylori-infected individuals.