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Lysozyme-inhibitory effect of wild-type and mutant Ivy. Micrococcus luteus was incubated in PBS alone (control) or in PBS containing HEWL in the presence or absence of rIvy, rIvyW55A, rIvyC82S, and rIvyH85D. Cell density was monitored by measuring the absorbance at 600 nm at various time points. Data are the means of three independent experiments and presented as means ± SEMs.
