Table 1.
Primers used in this study
| Primer | Sequence (5′ → 3′)a |
|---|---|
| F1 | GATATCGACGAGACTATTCCGCCCTCG (EcoRV) |
| R1 | GATATCTTTCCAATCCGGCTGCGAC (EcoRV) |
| F2 | CCGTCGGCGGTGCGCAG |
| R2 | GCACCGCCGACGGCAGATC |
| F3 | GCATGGTCAGCCAGCCGCACA |
| R3 | GGCTGGCTGACCATGCCGACC |
| F4 | GCCGGACAACTGCGGCAACC |
| R4 | CGCAGTTGTCCGGCTGGCA |
| F5 | GGATCCAGGGTTTTCGGTTTACT (BamHI) |
| R5 | CTATTTCCATAACGAGGGCGGAAT |
| F6 | CTCGTTATGGAAATAGCGCGC |
| R6 | GGATCCGTCAGCGATTTCACGA (BamHI) |
a
Underlined nucleotides are restriction sites of the enzymes indicated in the parentheses at the end of the sequence.