Fig 5.

Presence of E. coli gadAB is associated with attenuated host proinflammatory immune responses in octoassociated IL-10^−/− and WT mice. Expression of gadA (A) and gadB (B) in cecal E. coli from octoassociated mice. (C) Number of E. coli genomes per ng cecal content DNA. (D and E) Composite blinded histological inflammation scores of colonic segments (D) and spontaneous secretion of IL-12/23p40 in culture supernatants from proximal colon explants (E) of WT and IL-10^−/− mice octoassociated for 5 weeks with 7 bacteria and either NC101 or NC101ΔgadAB. Data are presented as the mean ± SEM (n = 5 mice per group). *, P < 0.05 relative to WT mice (A and B) or relative to octoassociated and NC101-monoassociated mice of the same genetic background (C to E).