Table 1.
Pneumococcal strains and plasmids used in the present study
| Strain or plasmid | Descriptiona | Reference(s) or source |
|---|---|---|
| Strains | ||
| D39 | Serotype 2, encapsulated | 63 |
| R6 | D39 derivative, unencapsulated | 64 |
| ST556 | Serotype 19F, OM clinical isolate | 34, 35 |
| ST606 | ST556 derivative with streptomycin resistance; Strepr | 34 |
| ST1733 | ST606 ΔarcDT::kan; Kanr | This study |
| ST1808 | ST606 ΔarcD::kan; Kanr | This study |
| ST1809 | ST606 ΔarcT::kan; Kanr | This study |
| ST1815 | R6 ΔarcD::kan; Kanr | This study |
| ST1898 | D39 ΔarcD::kan; Kanr | This study |
| ST1899 | D39 ΔarcT::kan; Kanr | This study |
| ST1900 | D39 ΔarcDT::kan; Kanr | This study |
| ST2611 | ST1898 (D39 ΔarcD) transformed with pST2605; Kanr Ermr | This study |
| ST2705 | ST1898 (D39 ΔarcD) transformed with pST2586; Kanr Ermr | This study |
| Plasmids | ||
| pVA838 | E. coli-S. pneumoniae shuttle vector; Ermr | 65 |
| pST2586 | gyrA promoter cloned between the BamHI and SalI sites of pVA838; Ermr | This study |
| pST2605 | D39 arcD ORF cloned at the BamHI site of pST2586; Ermr | This study |
a
Strepr, streptomycin resistance; Kanr, kanamycin resistance; Ermr, erythromycin resistance.