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Complementation of IApc35 and BC2 with the plasmid-borne mrkD1P restores biofilm resistance to PAO1 supernatant. Preformed biofilms of wild-type IA565 or mutant forms carrying either an empty vector (pBMC12) or mrkD1P complement (pBMC13) were treated with YEPD or PAO1 supernatant for 16 h, and remaining biofilm was assayed by crystal violet stain and OD570 reading.
