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Growth and AI-2 production in Y. pestis strain R88 (Δpgm) and the ΔluxS mutant. (A) Growth of strains with shaking in BHI broth at 30°C. (B) At each time point during growth, cell supernatants were collected, filtered, and assayed for AI-2 by using the V. harveyi MM32 reporter strain (see Materials and Methods). Data represent the means of two Y. pestis cultures per strain (A) and means ± standard deviations of four wells of MM32 medium per time point for two experiments (n = 8) (B).
