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Complementation of the ΔluxS mutant. Yersinia pestis strain R88, the ΔluxS mutant, and the complemented strain were grown with shaking in BHI broth at 30°C. At each time point during growth, cell supernatants were collected, filtered, and assayed for AI-2 by using the V. harveyi MM32 reporter strain. Data represent the means ± standard deviations of triplicate wells per time point and strain.
