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Comparison of AI-2 production in Y. pestis wild-type strain CO92 and strain R88 (Δpgm). Yersinia pestis strain R88 and wild-type strain CO92 were grown with shaking in BHI broth at 30°C in a BSL-3 facility. At each time point during growth, cell supernatants were collected, filtered, and assayed for AI-2 by using the V. harveyi MM32 reporter strain. Data represent the means ± standard deviations of triplicate wells per time point.
