Table 1.
Bacterial strains used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| BW25141 | Cloning strain | 9 |
| ER2566 | Overexpression strain | New England Biolabs |
| AH-pfs | ER2566/pET28a-pfs (Kanr) | This work |
| AH-luxS | ER2566/pET28a-luxS (Kanr) | This work |
| AH1070 | ER2566/pET28a-lsrB (Kanr) | This work |
| Yersinia pestis | ||
| CO92 | Yersinia pestis Lcr+ Pgm+ | 7 |
| R88 | CO92 Lcr+ Δpgm | R. D. Perry |
| R115 | CO92 Lcr+ Δpgm ΔypeIR ΔyspIR ΔluxS::kan | R. D. Perry |
| ISM1980 | R88 ΔluxS::kan | This work |
| YP21 | R88 ΔluxS::kan | This work |
| YP23 | R88 ΔluxS::frt/pJET1.2 (Ampr) | This work |
| YP21 | R88 ΔluxS::frt/pJET1.2-luxS | This work |
| YP24 | R88 Δlsr::frt | This work |
| Vibrio harveyi MM32 | luxN::cat luxS::Tn5kan | 12 |
| Plasmids | ||
| pET28a | Overexpression vector (Kanr) | Novagen |
| pET28a-pfs | Pfs expression vector (Kanr) | This work |
| pET28a-luxS | LuxS expression vector (Kanr) | This work |
| pET28a-lsrB | LsrB(Δ2–25) expression vector (Kanr) | This work |
| pJET1.2 | Cloning vector (Ampr) | Fermentas |
| pJET1.2-luxS | luxS complementation vector (Ampr) | This work |