Fig 2.
LtpD consists of two domains and associates via a 17-kDa internal region, LtpD472-626, with vesicles. LtpD or selected fragments of LtpD were ectopically expressed in HeLa cells. Cells were then stained with anti-Myc antibody (green) and DAPI DNA stain (blue). (A) Schematic overview of the LtpD fragments investigated in the present study. (B) Myc-LtpD localizes to large, vesicular structures around the nucleus. Analysis of fragments corresponding to the N- and C-terminal domains of LtpD shows that LtpD472-679 is sufficient to mediate association with vesicles. (C) Systematic analysis of fragments of the C-terminal domain of LtpD revealed that an internal domain, LtpD472-626, is required and sufficient for localization of LtpD to vesicles. Scale bars, 10 μm.