Table 1.
Bacterial strains used in this study
| Strain | Serogroup or genotypea | Source or reference |
|---|---|---|
| L. pneumophila | ||
| 130b (ATCC BAA-74) | O1; clinical isolate | 29 |
| 130b ΔdotA mutant | dotA gene disrupted with a Kanr cassette | 23 |
| 130b ΔltpD (ICC1064) mutant | lptD gene replaced with a Kanr cassette | This study |
| E. coli | ||
| BL21(DE3) | F− ompT hsdSB (rB− mB−) gal dcm | Novagen |
| Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 nupG recA1 araD139 Δ(ara-leu)7697 rpsL (Strr) endA1 λ− | Invitrogen |
a
Strr, streptomycin resistance; Kanr, kanamycin resistance.