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Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| 86-24 | Wild-type Stx2+ EHEC strain (serotype O157:H7) | 67 |
| YNN01 | 86-24 Δstx2a | This study |
| YNN02 | YNN01 λPR | This study |
| YNN03 | YNN01λPR-yenI | This study |
| Plasmids | ||
| pKD3 | λ-Red template plasmid | 51 |
| pKD46 | λ-Red helper plasmid (recombinase) | 51 |
| pCP20 | λRed resolvase plasmid | 51 |
| pJLD1600 | λPR-yenI-FRT-kan-FRT template plasmid | 36 |
| pJLD500 | λPR-FRT-kan-FRT vector template plasmid | 36 |
